Fig. 6. Demonstration of percentage viable luteal cells treated with increasing concentrations of Thrombospondin1 (50-500 ng/ml) and also for TSP1 knockout luteal cells for 72 hour at 37⁰C. MTT was added at 5 mg/ml to each well and further incubated for 4 h and absorbance was measured at 450 nm using a 96-plate reader to measure cell proliferation. Abbreviation: MTT, 3-(4,5-dimethylthiazole-2-yl))-2-5-diphenyltetrazolium bromide; TSP, Thrombospondin; TSP1-KO, TSP1 knockout.